1U9Y
Crystal Structure of Phosphoribosyl Diphosphate Synthase from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.011, 138.280, 138.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Bacillus subtilis PRPP synthase |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.336 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.081 | 0.415 |
| Number of reflections | 40242 | |
| <I/σ(I)> | 20.7 | 4.1 |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
