1U99
Crystal Structures of E. coli RecA in a Compressed Helical Filament Form 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | SBC-2 |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 61 |
| Unit cell lengths | 108.001, 108.001, 74.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.180 - 2.600 |
| Rwork | 0.213 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 2REB |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.180 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.071 | 0.823 |
| Number of reflections | 14222 | |
| <I/σ(I)> | 50.9 | 3.8 |
| Completeness [%] | 98.7 | 98.1 |
| Redundancy | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | PEG 200, sodium citrate, sodium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
