1U94
Crystal Structure of E. Coli RecA in a Compressed Helical Filament Form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-30 |
| Detector | SBC-2 |
| Spacegroup name | P 61 |
| Unit cell lengths | 105.854, 105.854, 73.496 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.650 - 1.900 |
| Rwork | 0.187 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2reb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.650 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.713 |
| Number of reflections | 35211 | |
| <I/σ(I)> | 36.3 | 3.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG 550 MME, calcium chloride, hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
