1U83
PSL synthase from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-07-29 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97945, 0.97921 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 92.071, 92.071, 92.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.040 - 2.200 |
| R-factor | 0.1922 |
| Rwork | 0.188 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.040 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 13466 | |
| <I/σ(I)> | 12.9 | 3.17 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | Ammonium phosphate, Bis-Tris, ethylene glycol, glycerol, pH 7.0, VAPOR DIFFUSION, temperature 298K |
