1U7P
X-ray Crystal Structure of the Hypothetical Phosphotyrosine Phosphatase MDP-1 of the Haloacid Dehalogenase Superfamily
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-19 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.524, 57.651, 76.621 |
| Unit cell angles | 90.00, 91.97, 90.00 |
Refinement procedure
| Resolution | 76.580 - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HIGH RESOLUTION MODEL OF MDP-1 WITHOUT MAGNESIUM BOUND |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.580 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 48167 | |
| <I/σ(I)> | 19.6 | 2.8 |
| Completeness [%] | 93.8 | 88.3 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 291 | PEG 3350, sodium acetate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.00 |
