1U7N
Crystal Structure of the fatty acid/phospholipid synthesis protein PlsX from Enterococcus faecalis V583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-22 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.362, 82.423, 147.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.520 - 2.260 |
| R-factor | 0.2089 |
| Rwork | 0.206 |
| R-free | 0.26178 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.443 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.520 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.127 | 0.640 |
| Number of reflections | 36201 | |
| <I/σ(I)> | 5.4 | 2.1 |
| Completeness [%] | 99.4 | 94.6 |
| Redundancy | 10.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Ethanol, HEPES, MgCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
