1U7H
Structure and a Proposed Mechanism for Ornithine Cyclodeaminase from Pseudomonas putida
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-07 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.97946, 0.97888, 0.96114 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.900, 78.600, 119.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.950 - 1.800 |
| Rwork | 0.158 |
| R-free | 0.18100 |
| Structure solution method | MAD |
| Starting model (for MR) | EXPERIMENTAL PHASES |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 62180 | |
| <I/σ(I)> | 39.4 | 7.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.6 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.25 | 293 | 0.1M Na-MES and 40% MPD, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
