1U7D

crystal structure of apo M. jannashii tyrosyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	45.119, 185.292, 95.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.680 - 2.650
R-factor	0.229
Rwork	0.227
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j1u
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.720
High resolution limit [Å]	2.650	2.650
Number of reflections	22575
<I/σ(I)>	21.5	2.1
Completeness [%]	94.2	96
Redundancy	3.5	3.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	5.5	277	12-15% PEG4000, 0.2M ammonium sulfate, 0.2M sodium acetate, , pH 5.5, LIQUID DIFFUSION, temperature 277K