1U79
Crystal structure of AtFKBP13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-05 |
| Detector | BRANDEIS |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.026, 126.606, 119.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yat |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.950 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.220 |
| Number of reflections | 44839 | |
| <I/σ(I)> | -3 | |
| Completeness [%] | 93.6 | 43.7 |
| Redundancy | 3.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 295 | 8-11% PEG 550MME, 2.5M ammonium sulfate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
