1U75
Electron Transfer Complex between Horse Heart Cytochrome c and Zinc-Porphyrin Substituted Cytochrome c Peroxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2003-03-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9363 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 104.465, 104.465, 186.884 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.550 |
| R-factor | 0.27303 |
| Rwork | 0.271 |
| R-free | 0.30632 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pcb |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 34216 | |
| <I/σ(I)> | 10.4 | 3.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
