1U71
Understanding the Role of Leu22 Variants in Methotrexate Resistance: Comparison of Wild-type and Leu22Arg Variant Mouse and Human Dihydrofolate Reductase Ternary Crystal Complexes with Methotrexate and NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-07-18 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | H 3 |
| Unit cell lengths | 85.144, 85.144, 78.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.211 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | wild type hDHFR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.059 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PROTEIN |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | |
| Number of reflections | 6383 | |
| Completeness [%] | 87.1 | 60.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 62% AS, phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
