1U6T
Crystal structure of the human SH3 binding glutamic-rich protein like
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.886, 34.815, 97.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.380 - 1.900 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.21200 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD (& SHARP) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.380 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.120 |
| Number of reflections | 8126 | |
| <I/σ(I)> | 9.1 | 4.9 |
| Completeness [%] | 98.0 | 85.2 |
| Redundancy | 3.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | tri-Sodium Citrate dihydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
