1U53
Novel X-Ray Structure of Na-ASP-2, a PR-1 protein from the nematode parasite Necator americanus and a vaccine antigen for human hookworm infection
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-16 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.890, 51.540, 43.630 |
| Unit cell angles | 90.00, 114.06, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.560 |
| R-factor | 0.17777 |
| Rwork | 0.175 |
| R-free | 0.22355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.678 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.620 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.154 | |
| Number of reflections | 17644 | |
| <I/σ(I)> | 27.6 | 3.5 |
| Completeness [%] | 80.9 | 10.7 |
| Redundancy | 1.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 100mM Imidazole, 40% PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
