1U4C
Structure of spindle checkpoint protein Bub3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-04-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97945, 0.97958, 0.9200 |
| Spacegroup name | P 32 |
| Unit cell lengths | 73.266, 73.266, 110.425 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.350 |
| R-factor | 0.202 |
| Rwork | 0.196 |
| R-free | 0.28800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 52125 | |
| <I/σ(I)> | 19 | 1.7 |
| Completeness [%] | 94.6 | 93.2 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 296 | 20% PEG 3350, 100mM citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
