1U42
Crystal structure of MLAM mutant of dimerisation domain of NF-kB p50 transcription factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.864 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 59.403, 59.403, 59.856 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.260 - 2.699 |
R-factor | 0.265 |
Rwork | 0.259 |
R-free | 0.31700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bfs |
RMSD bond length | 0.009 |
RMSD bond angle | 1.590 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.260 | 2.760 |
High resolution limit [Å] | 2.699 | 2.699 |
Rmerge | 0.347 | |
Number of reflections | 3233 | |
<I/σ(I)> | 17.5 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | PEG 8000, ammonium sulphate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |