1U22
A. thaliana cobalamine independent methionine synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979667 |
| Spacegroup name | P 65 |
| Unit cell lengths | 122.423, 122.423, 131.365 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.780 - 2.650 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.27000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1u1h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 32422 | |
| <I/σ(I)> | 23 | 4.93 |
| Completeness [%] | 99.5 | 97.9 |
| Redundancy | 11.2 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 30% (W/V) POLY-ETHYLENE GLYCOL (PEG) 8000, 0.2 M AMMONIUM SULFATE, 1 MM DTT, 0.1 M SODIUM CACODYLATE, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
