1U1Z
The Structure of (3R)-hydroxyacyl-ACP dehydratase (FabZ)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.031, 100.897, 177.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.350 - 2.500 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.25800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNX (2000.1) |
| Refinement software | CNX (2000.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.500 |
| Number of reflections | 108745 | |
| <I/σ(I)> | 17.1 | 3 |
| Completeness [%] | 97.0 | 90.9 |
| Redundancy | 5.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Ammonium Sulphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
