1U1Q
Crystal Structure of UP1 Complexed With d(TTAGGGTTA(DI)GG); A Human Telomeric Repeat Containing Inosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2004-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.502, 50.502, 172.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2up1 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.032 | 0.119 |
| Number of reflections | 21024 | |
| <I/σ(I)> | 46.5 | 15.3 |
| Completeness [%] | 97.3 | 92.1 |
| Redundancy | 6.17 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 283 | ammonium phosphate, glycerol, Tris, sodium chloride, MES, EDTA, beta-mercaptoethanol , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 283.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium phosphate | ||
| 10 | 1 | 2 | sodium chloride | ||
| 11 | 1 | 2 | MES | ||
| 12 | 1 | 2 | EDTA | ||
| 13 | 1 | 2 | beta-mercaptoethanol | ||
| 14 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | sodium chloride | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 1 | EDTA | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | ammonium phosphate | ||
| 8 | 1 | 2 | glycerol | ||
| 9 | 1 | 2 | Tris |
