1U1H
A. thaliana cobalamine independent methionine synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 |
| Unit cell lengths | 121.725, 121.725, 132.707 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.610 - 2.550 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.560 |
| Number of reflections | 68990 | |
| <I/σ(I)> | 10.295 | 1.081 |
| Completeness [%] | 96.6 | 96 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 30% (W/V) POLY-ETHYLENE GLYCOL (PEG) 8000, 0.2 M AMMONIUM SULFATE, 1 MM DTT AND 0.1 M SODIUM CACODYLATE, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
