1U16
Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM) in complex with sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 133.280, 133.280, 73.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.160 - 2.200 |
| Rwork | 0.212 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code:1HY1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.160 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 34371 | |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | ammonium sulfate, cobalt chloride, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
