1U0L
Crystal structure of YjeQ from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.819, 114.798, 77.088 |
| Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.28400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 29551 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 15% PEG3350, 0.2M KCL, 10mM Guanidine HCl, 0.1M Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
