1U0D

Y33H Mutant of Homing endonuclease I-CreI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Collection date	2003-08-04
Spacegroup name	P 1
Unit cell lengths	43.838, 70.331, 88.016
Unit cell angles	90.28, 89.94, 98.78

Refinement procedure

Resolution	29.500 - 2.900
R-factor	0.242
Rwork	0.217
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	29.500
High resolution limit [Å]	2.900
Number of reflections	21529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1