1U07
Crystal Structure of the 92-residue C-term. part of TonB with significant structural changes compared to shorter fragments
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 22.580, 49.320, 72.220 |
| Unit cell angles | 90.00, 97.99, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.130 |
| R-factor | 0.139 |
| Rwork | 0.137 |
| R-free | 0.18300 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.037 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.100 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.072 | 0.519 |
| Number of reflections | 65348 | |
| <I/σ(I)> | 12.68 | 1.51 |
| Completeness [%] | 99.8 | 78.5 |
| Redundancy | 6.54 | 1.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 100mM imidazole, 1.1M sodium citrate, 100mM NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
