1U06
crystal structure of chicken alpha-spectrin SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.006231 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.600, 42.250, 49.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.110 - 1.490 |
| R-factor | 0.1757 |
| Rwork | 0.175 |
| R-free | 0.18850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.531 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.550 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Number of reflections | 11318 | |
| <I/σ(I)> | 23.5 | 5.3 |
| Completeness [%] | 93.9 | 79.9 |
| Redundancy | 6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
