1TZZ
Crystal structure of the protein L1841, unknown member of enolase superfamily from Bradyrhizobium japonicum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-07-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97958,0.97935,0.96411 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.975, 178.003, 110.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.860 |
| R-factor | 0.238 |
| Rwork | 0.234 |
| R-free | 0.25380 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.260 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 78930 | |
| Completeness [%] | 99.3 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 3350, Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
