1TZY
Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 |
| Unit cell lengths | 158.351, 158.351, 103.576 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.960 - 1.900 |
| R-factor | 0.18844 |
| Rwork | 0.187 |
| R-free | 0.22152 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.923 |
| Data reduction software | PXGEN |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.960 | 1.948 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.089 | |
| Number of reflections | 109956 | |
| <I/σ(I)> | 25.7 | |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.7 | 277 | 2.0M KCL, 1.35M PHOSPHATE, pH 6.7, MICRODIALYSIS, temperature 277K |
