1TZN
Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore bound to the VWA domain of CMG2, an anthrax toxin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9186 |
| Spacegroup name | P 1 |
| Unit cell lengths | 158.800, 158.846, 214.084 |
| Unit cell angles | 69.58, 69.07, 65.58 |
Refinement procedure
| Resolution | 20.000 - 4.300 |
| R-factor | 0.325 |
| Rwork | 0.322 |
| R-free | 0.33000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3.6 heptameric prepore model |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.450 |
| High resolution limit [Å] | 4.300 | 4.300 |
| Number of reflections | 120016 | |
| <I/σ(I)> | 6.8 | 1.8 |
| Completeness [%] | 97.3 | 98.1 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 8000, sodium chloride, Tris, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
