1TZL
Crystal Structure of Pyranose 2-Oxidase from the White-Rot Fungus Peniophora sp.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97934, 0.97950, 0.93926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 164.879, 103.596, 169.169 |
| Unit cell angles | 90.00, 105.25, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.350 |
| R-factor | 0.17604 |
| Rwork | 0.174 |
| R-free | 0.22575 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.450 |
| Data scaling software | XDS |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.092 | 0.295 |
| Number of reflections | 228044 | |
| <I/σ(I)> | 8.42 | 3.99 |
| Completeness [%] | 96.1 | 94.9 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 6000, MES, tributyl phosphine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
