1TZI

Crystal Structure of the Fab YADS2 Complexed with h-VEGF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	96.504, 149.588, 117.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.2197
Rwork	0.218
R-free	0.25447
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The VEGF is from pdb entry 1VPF. The Fab is from an in-house determined structure.
RMSD bond length	0.011
RMSD bond angle	1.334
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.076	0.400
Number of reflections	20861
<I/σ(I)>	18.7	2.5
Completeness [%]	97.3	82.92
Redundancy	4.8	83.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	292	LITHIUM CHLORIDE, PEG 6000, MES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 292K