1TZE

SIGNAL TRANSDUCTION ADAPTOR GROWTH FACTOR, GRB2 SH2 DOMAIN COMPLEXED WITH PHOSPHOTYROSYL HEPTAPEPTIDE LYS-PRO-PHE-PTYR-VAL-ASN-VAL-NH2 (KFPPYVNC-NH2)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-10-03
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	50.300, 50.300, 89.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.203
Rwork	0.203
RMSD bond length	0.012
RMSD bond angle	24.926 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.050
Total number of observations	45126 *
Number of reflections	7237
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-25 (mg/ml)
2	1	drop	ammonium phosphate	1.5 (M)
3	1	drop	sodium cacodylate	50 (mM)