1TZ8
The monoclinic crystal structure of transthyretin in complex with diethylstilbestrol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.271, 96.961, 81.717 |
| Unit cell angles | 90.00, 107.94, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f86 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.326 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.370 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.064 | 0.267 |
| Number of reflections | 46580 | |
| <I/σ(I)> | 9.8 | 2.5 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 287 | Sodium Acetate (0.2M), Ammonium Sulfate (2.4M), glycerol (7%), pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
