1TZ7
Aquifex aeolicus amylomaltase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 156.745, 156.745, 169.161 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.150 |
R-factor | 0.21608 |
Rwork | 0.215 |
R-free | 0.24256 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Thermus thermophilus amylomaltase |
RMSD bond length | 0.009 |
RMSD bond angle | 0.686 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.059 | 0.727 |
Number of reflections | 75895 | |
Completeness [%] | 91.7 | 96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2-methyl-2,4-pentanediol, ammonium sulfate, Tris/HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |