1TZ0
Crystal Structure of Putative Antibiotic Biosythesis Monooxygenase from Bacillus cereus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-20 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9793, 0.9794, 0.954 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.894, 86.080, 42.867 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 37.270 - 1.840 |
| R-factor | 0.20962 |
| Rwork | 0.204 |
| R-free | 0.26073 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.333 |
| Data reduction software | CrystalClear (D*TRECK (MSC/RIGAKU)) |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.083 | 0.479 |
| Number of reflections | 38958 | |
| <I/σ(I)> | 9.3 | 2.3 |
| Completeness [%] | 99.4 | 95 |
| Redundancy | 6.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | Sodium Formate, Sodium Acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
