1TYB
STRUCTURAL ANALYSIS OF A SERIES OF MUTANTS OF TYROSYL-TRNA SYNTHETASE: ENHANCEMENT OF CATALYSIS BY HYDROPHOBIC INTERACTIONS
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.460, 64.460, 237.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.23 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.032 |
| Refinement software | PROLSQ |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 0.125 (%(w/v)) | or 0.25 %(w/v) |
| 3 | 1 | drop | 0.01 (M) | ||
| 4 | 1 | drop | Tris-HCl | 0.01 (M) | |
| 5 | 1 | reservoir | PEG8000 | 0.25 (%(w/v)) | or 0.5 %(w/v) |
| 6 | 1 | reservoir | Tris-HCl | 0.01 (M) |
