1TWI

Crystal structure of Diaminopimelate Decarboxylase from m. jannaschii in co-complex with L-lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-04
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	70.242, 147.070, 89.389
Unit cell angles	90.00, 97.25, 90.00

Refinement procedure

Resolution	29.250 - 2.000
R-factor	0.157
Rwork	0.157
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tuf
RMSD bond length	0.021
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	119738
<I/σ(I)>	35.37	13.06
Completeness [%]	99.1	100
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	12% PEG6000, 0.1M Tris-HCl, pH 8.0, 20mM MgCl2, 10mM L-lysine, VAPOR DIFFUSION, HANGING DROP, temperature 291K