1TV4
Crystal structure of the sulfite MtmB complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.82700 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 158.820, 158.820, 136.333 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.850 - 1.800 |
R-factor | 0.174 |
Rwork | 0.174 |
R-free | 0.18700 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1nth |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.065 | 0.347 |
Number of reflections | 92028 | |
<I/σ(I)> | 23.7 | 3.7 |
Completeness [%] | 98.3 | 95.9 |
Redundancy | 5.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | NACL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |