1TU4
Crystal Structure of Rab5-GDP Complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9798,0.9794,0.9253 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.900, 84.900, 199.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.610 - 2.200 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.27400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | 0.378 |
| Number of reflections | 42778 | |
| <I/σ(I)> | 32.6 | 3.4 |
| Completeness [%] | 98.8 | 89.6 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | ammonium sulfate, cobalt chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
