1TT1
CRYSTAL STRUCTURE OF THE GLUR6 LIGAND BINDING CORE IN COMPLEX WITH KAINATE 1.93 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.682, 106.228, 57.665 |
| Unit cell angles | 90.00, 101.38, 90.00 |
Refinement procedure
| Resolution | 24.950 - 1.930 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.950 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.074 | 0.349 |
| Number of reflections | 41047 | |
| <I/σ(I)> | 10.6 | 4.07 |
| Completeness [%] | 99.3 | 92.8 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | streak seeding | 4 | 297 | 18% PEG 3350, 25 mM Citrate, 2 mM Malonate, 20 mM NaCl, 1 mM EDTA, 10 mM BME, pH 4.0, streak seeding, temperature 297K |
