1TSU
CRYSTAL STRUCTURE OF DECAMER NCP1 SUBSTRATE PEPTIDE IN COMPLEX WITH WILD-TYPE D25N HIV-1 PROTEASE VARIANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 220 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-29 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.058, 57.731, 61.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.260 - 2.100 |
| R-factor | 0.20717 |
| Rwork | 0.206 |
| R-free | 0.23355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mtr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.462 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.260 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.092 |
| Number of reflections | 10450 |
| Completeness [%] | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | SODIUM PHOSPHATE, SODIUM CITRATE, AMMONIUM SULPHATE, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
