1TR5
Room temperature structure of Staphylococcal nuclease variant truncated Delta+PHS I92E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.298, 60.981, 38.789 |
| Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
| Resolution | 25.590 - 2.100 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tqo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.590 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.153 |
| Number of reflections | 8362 | |
| <I/σ(I)> | 18.72 | 4.25 |
| Completeness [%] | 97.9 | 96.4 |
| Redundancy | 4.17 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | MPD, potassium phosphate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
