1TQY
The Actinorhodin Ketosynthase/Chain Length Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-11-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.127 |
| Spacegroup name | H 3 |
| Unit cell lengths | 262.700, 262.700, 224.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.233 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FabF dimer generated from 1KAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.310 |
| Number of reflections | 384406 | |
| <I/σ(I)> | 9.3 | 1.8 |
| Completeness [%] | 98.7 | 95.5 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
