1TQM
Crystal Structure of A. fulgidus Rio2 Serine Protein Kinase Bound to AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.165, 44.259, 62.097 |
| Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
| Resolution | 28.990 - 1.990 |
| R-factor | 0.17435 |
| Rwork | 0.171 |
| R-free | 0.22775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tqi |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 20313 | |
| <I/σ(I)> | 40.9 | 12.1 |
| Completeness [%] | 95.7 | |
| Redundancy | 7.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.83 | 293 | PEG 900, SODIUM PHOSPHATE, SODIUM CITRATE, pH 3.83, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
