1TQE
Mechanism of recruitment of class II histone deacetylases by myocyte enhancer factor-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.797, 66.930, 66.967 |
| Unit cell angles | 76.67, 71.83, 71.81 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| Rwork | 0.263 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Structure of MEF2A and DNA complex pdb entry 1egw |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.040 | 0.227 |
| Number of reflections | 17405 | |
| <I/σ(I)> | 63 | 5.5 |
| Completeness [%] | 91.3 | 95 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.35 | 315 | BTP, PEG, NaCl, glycerol, MgCl2, CaCl2, pH 6.35, VAPOR DIFFUSION, HANGING DROP, temperature 315K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | BTP | ||
| 2 | 1 | 1 | PEG | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | NaCl | ||
| 5 | 1 | 2 | glycerol | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | CaCl2 | ||
| 8 | 1 | 2 | H2O |
