1TQD

Crystal structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-02-01
Detector	MARRESEARCH
Wavelength(s)	0.9786, 0.9789, 0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	83.071, 93.670, 139.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.236
Rwork	0.214
R-free	0.25800
Structure solution method	AB INITIO PHASING
RMSD bond length	0.007
RMSD bond angle	1.320
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	97950
<I/σ(I)>	13.6	5.5
Completeness [%]	98.8	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	PEG 20000, MES, Ethanol, pH 6.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	6.5	293	PEG 20000, MES, Ethanol, pH 6.5, VAPOR DIFFUSION, temperature 293K