1TQ4

Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-17
Detector	MARRESEARCH
Wavelength(s)	0.986
Spacegroup name	P 21 21 21
Unit cell lengths	68.700, 71.000, 87.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.950
R-factor	0.211
Rwork	0.193
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	31715
<I/σ(I)>	24.3	7.9
Completeness [%]	99.0	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	293	PEG 4000, NaOAc, pH 4.6, VAPOR DIFFUSION, temperature 293K