1TPT

THREE-DIMENSIONAL STRUCTURE OF THYMIDINE PHOSPHORYLASE FROM ESCHERICHIA COLI AT 2.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	133.000, 133.000, 67.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.800
R-factor	0.28
RMSD bond length	0.011
RMSD bond angle	1.500
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Total number of observations	77819 *
Number of reflections	15034 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *		took from Cook, W.J.,(1987) J. Biol. Chem., 262, 3788. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	52 (mg/ml)
2	1	drop	ammonium sulfate	35 (%)
3	1	drop	citrate	0.05 (M)
4	1	reservoir	ammonium sulfate	35 (%)
5	1	reservoir	citrate	0.05 (M)