1TP5

Crystal structure of PDZ3 domain of PSD-95 protein complexed with a peptide ligand KKETWV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-23
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 41 3 2
Unit cell lengths	89.978, 89.978, 89.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.650 - 1.540
R-factor	0.1929
Rwork	0.191
R-free	0.22926
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1be9
RMSD bond length	0.017
RMSD bond angle	1.558
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.630
High resolution limit [Å]	1.540	1.540
Number of reflections	18320
Completeness [%]	96.2	95.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	288	1.0 M sodium citrate, 0.1 M HEPES, 10 mM sodium chloride, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 288K