1TOO

Interleukin 1B Mutant F146W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1999-11-11
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	54.950, 54.950, 77.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.250 - 2.100
R-factor	0.186
Rwork	0.185
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	77.250
High resolution limit [Å]	2.100
Number of reflections	13286
Completeness [%]	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	29% AMMONIUM SULFATE W/V, 100 MM Tris , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K