1TO5
Structure of the cytosolic Cu,Zn SOD from S. mansoni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-19 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5408 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.640, 78.240, 95.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.800 - 2.200 |
| Rwork | 0.176 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 25229 | |
| <I/σ(I)> | 17.3 | 6.3 |
| Completeness [%] | 86.5 | 72.9 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 30% PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
