1TNZ

Rat Protein Geranylgeranyltransferase Type-I Complexed with a GGPP analog and a RRCVLL Peptide Derived from Cdc42 splice isoform-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1000
Spacegroup name	C 1 2 1
Unit cell lengths	271.262, 266.922, 185.750
Unit cell angles	90.00, 131.91, 90.00

Refinement procedure

Resolution	29.960 - 2.900
R-factor	0.199
Rwork	0.199
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdn entry 1N4Q
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	197897
<I/σ(I)>	11.9	2.1
Completeness [%]	91.4	81
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	1.3 M ammonium sulfate, 175 mM NaCitrate pH 6.5, 100 mM MES pH 6.3, 20 mM DTT, 1uM ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K